Department of BioMedical Engineering, University Medical Center Groningen and University of Groningen, The Netherlands.
Biofouling. 2010 Oct;26(7):761-7. doi: 10.1080/08927014.2010.515027.
The fate of secondary biomaterial implants was determined by bio-optical imaging and plate counting, after antibiotic treatment of biomaterials-associated-infection (BAI) and surgical removal of an experimentally infected, primary implant. All primary implants and tissue samples from control mice showed bioluminescence and were culture-positive. In an antibiotic treated group, no bioluminescence was detected and only 20% of all primary implants and no tissue samples were culture-positive. After revision surgery, bioluminescence was detected in all control mice. All the implants and 80% of all tissue samples were culture-positive. In contrast, in the antibiotic treated group, 17% of all secondary implants and 33% of all tissue samples were culture-positive, despite antibiotic treatment. The study illustrates that due to the BAI of a primary implant, the infection risk of biomaterial implants is higher in revision surgery than in primary surgery, emphasizing the need for full clearance of the infection, as well as from surrounding tissues prior to implantation of a secondary implant.
采用生物光学成像和平板计数法来确定二次生物材料植入物的命运，方法是对生物材料相关感染（BAI）进行抗生素治疗，并对实验性感染的原发性植入物进行手术切除。所有原发性植入物和对照小鼠的组织样本均显示生物发光且培养阳性。在抗生素治疗组中，未检测到生物发光，并且所有原发性植入物中只有 20%和没有组织样本培养阳性。在翻修手术后，所有对照小鼠均检测到生物发光。所有植入物和 80%的所有组织样本均培养阳性。相比之下，在抗生素治疗组中，尽管进行了抗生素治疗，但所有二次植入物中有 17%和所有组织样本中有 33%为培养阳性。该研究表明，由于原发性植入物的 BAI，在翻修手术中生物材料植入物的感染风险高于初次手术，这强调了在植入二次植入物之前，需要完全清除感染以及周围组织。
