Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.
Luminescence. 2011 Sep-Oct;26(5):342-8. doi: 10.1002/bio.1235. Epub 2010 Aug 25.
A new, simple and sensitive spectrofluorimetric method has been developed for the determination of pregabalin (PG) in capsules. The method is based on the reaction between pregabalin and fluorescamine in borate buffer solution of pH 10 to give a highly fluorescent derivative that is measured at 487 nm after excitation at 390 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The fluorescence intensity concentration plot was rectilinear over the range of 0.01-0.3 µg mL⁻¹ with a lower detection limit of 0.0017 µg mL⁻¹ and limit of quantitation of 0.005 µg mL⁻¹. The developed method was successfully applied to the analysis of the drug in its commercial capsules. The mean percentage recovery of PG in its capsule was 99.93±1.24 (n = 3). Statistical comparison of the results with those of the comparison method revealed good agreement and proved that there was no significant difference in the accuracy and precision of the two methods. A proposed reaction pathway was postulated.
一种新的、简单且灵敏的荧光分光光度法已被开发用于测定胶囊中的普瑞巴林（PG）。该方法基于普瑞巴林与荧光胺在硼酸盐缓冲溶液（pH 10）中的反应，生成一种高度荧光的衍生物，在 390nm 激发下于 487nm 处测量。仔细研究并优化了影响反应产物形成和稳定性的不同实验参数。荧光强度浓度图在 0.01-0.3μgmL⁻¹范围内呈线性，检测限为 0.0017μgmL⁻¹，定量限为 0.005μgmL⁻¹。所开发的方法成功地应用于其商业胶囊中药物的分析。PG 在其胶囊中的平均回收率为 99.93±1.24（n = 3）。与比较方法的结果进行统计学比较表明，两种方法的准确性和精密度没有显著差异，证明了这一点。提出了一个反应途径。
