Funabiki K, Horikoshi S, Tomino Y, Nagai Y, Koide H
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Clin Nephrol. 1990 Dec;34(6):239-46.
Immunofluorescence and immunoperoxidase staining was carried out to determine correlations between the progression of glomerular sclerosis and changes in the amount or distribution of glomerular extracellular components, including type I, III, IV, and VI collagens, laminin and fibronectin, in patients with IgA nephropathy, membranoproliferative glomerulonephritis and rapidly progressive glomerulonephritis. Staining of type I and III collagens was not observed in glomeruli from normal individuals or patients with mild glomerulonephritis. In the advanced stages of glomerulonephritis, the staining of type IV and VI collagens, laminin and fibronectin was marked in the glomerular mesangium, and the distribution of fibronectin extended to the glomerular capillary walls in the sclerotic lesions of glomeruli. However, the staining intensity of type IV collagen, laminin and fibronectin was gradually decreased during the progression of glomerular sclerosis. On the other hand, the staining of type I and III collagens was observed focally in sclerotic or hyalinotic glomeruli and around such glomeruli in those patients. Light microscopic examination revealed that patients who showed marked staining of type I and III collagens by immunofluorescence had severe damage of Bowman's capsules. These results suggest that the hyperproduction and/or invasion of interstitial collagens, i.e., types I and III, are closely linked to the progression of glomerular sclerosis and hyalinosis in patients with various types of glomerulonephritis.
采用免疫荧光和免疫过氧化物酶染色法，以确定IgA肾病、膜增生性肾小球肾炎和快速进行性肾小球肾炎患者肾小球硬化的进展与肾小球细胞外成分（包括I型、III型、IV型和VI型胶原、层粘连蛋白和纤连蛋白）数量或分布变化之间的相关性。在正常个体或轻度肾小球肾炎患者的肾小球中未观察到I型和III型胶原的染色。在肾小球肾炎的晚期，IV型和VI型胶原、层粘连蛋白和纤连蛋白在肾小球系膜中染色明显，并且在肾小球硬化病变中，纤连蛋白的分布延伸至肾小球毛细血管壁。然而，在肾小球硬化进展过程中，IV型胶原、层粘连蛋白和纤连蛋白的染色强度逐渐降低。另一方面，在这些患者的硬化或透明变性肾小球及其周围局部观察到I型和III型胶原的染色。光镜检查显示，免疫荧光显示I型和III型胶原染色明显的患者，其鲍曼囊有严重损伤。这些结果表明，间质胶原即I型和III型胶原的过度产生和/或浸润与各种类型肾小球肾炎患者的肾小球硬化和透明变性进展密切相关。
