Madsen K, Holmskov U
Department of Physical Education, Odense University, Denmark.
Eur J Appl Physiol Occup Physiol. 1995;71(5):472-4. doi: 10.1007/BF00635884.
An immunohistochemical method, based on an indirect peroxidase method for staining of capillaries containing collagen type IV, is presented. Biopsies from the vastus lateralis muscle of eight endurance trained runners were stained and counted for capillaries with the collagen type IV method and with the widely used amylase-PAS method. The mean number +/- SEM of capillaries per fibre was 2.48 +/- 0.09 with the collagen type IV method and 2.41 +/- 0.09 with the collagen type IV method and 2.41 +/- 0.08 with the amylase method. The 95% confidence intervals for the differences between the two methods were - 0.03 to 0.15. We conclude that the collagen type IV immunohistochemical method for capillary staining is comparable with the amylase method under optimum conditions, but that it is likely to be more dependable. It also has the advantage that it reveals fibre outlines.
本文介绍了一种免疫组织化学方法，该方法基于间接过氧化物酶法对含IV型胶原的毛细血管进行染色。对8名耐力训练的跑步者的股外侧肌活检组织进行染色，并用IV型胶原方法和广泛使用的淀粉酶-过碘酸雪夫氏（PAS）法对毛细血管进行计数。采用IV型胶原方法时，每根纤维的毛细血管平均数量±标准误为2.48±0.09，采用淀粉酶法时为2.41±0.08。两种方法差异的95%置信区间为-0.03至0.15。我们得出结论，在最佳条件下，IV型胶原免疫组织化学毛细血管染色方法与淀粉酶法相当，但可能更可靠。它还有一个优点，即能显示纤维轮廓。
