Statens Serum Institut, Copenhagen S, Denmark.
J Appl Microbiol. 2010 Dec;109(6):2032-8. doi: 10.1111/j.1365-2672.2010.04833.x.
To characterize isolates of Salmonella Typhimurium DT41 obtained from infected flocks of broiler breeders by multiple-locus variable-number tandem-repeats analysis (MLVA) and compare results with a diverse strain collection from Germany and United Kingdom and isolates from Danish patients.
A total of 102 isolates of Salm. Typhimurium phage type DT41 were MLVA typed. MLVA typing showed 4, 12, 25, 9 and 8 different alleles at the five MLVA loci 9, 5, 6, 10 and 3, respectively. A dendrogram based on MLVA types was constructed, and one large group, nine minor groups and 29 more unrelated MLVA types were obtained. The major group included 20 of the 30 human isolates. Isolates obtained from broiler breeders demonstrated major diversity, indicating the existence of several independent introductions of DT41 at farm level. When comparison was made to isolates included from Germany and England, DT41 seems to be ubiquitous in the wild fauna which might represent a risk factor for poultry.
Transmission from Danish broilers to humans was not demonstrated, neither was the transmission from rearing farms to broiler breeder farms. Sources of infection at broiler breeder farm level remained unidentified.
Major diversity was demonstrated for DT41 MLVA types. A persisting problem with infection of broiler breeder flocks with DT41 was not reflected in broiler flocks originating from these flocks.
通过多位点可变数目串联重复分析（MLVA）对来自受感染的肉鸡种鸡群的肠炎沙门氏菌 DT41 分离株进行特征描述，并将结果与来自德国和英国的多样化菌株集和丹麦患者的分离株进行比较。
对 102 株肠炎沙门氏菌 DT41 噬菌体型分离株进行了 MLVA 分型。MLVA 分型显示在五个 MLVA 基因座 9、5、6、10 和 3 上分别有 4、12、25、9 和 8 个不同的等位基因。基于 MLVA 型构建了一个系统发育树，得到了一个大组、九个小组和 29 个不相关的 MLVA 型。主要组包括 30 个人类分离株中的 20 个。从肉鸡种鸡中获得的分离株显示出主要的多样性，表明在农场水平上存在几种独立的 DT41 引入。与来自德国和英国的分离株进行比较时，DT41 似乎在野生动物中普遍存在，这可能是家禽的一个风险因素。
未证明 DT41 从丹麦肉鸡传播给人类，也未证明从饲养场传播给肉鸡种鸡场。种鸡场水平的感染源仍未确定。
肠炎沙门氏菌 DT41 MLVA 型表现出高度多样性。持续存在的肠炎沙门氏菌 DT41 感染肉鸡种鸡群的问题并未反映在源自这些鸡群的肉鸡群中。
