Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221-0006, USA.
J Eukaryot Microbiol. 2010 Sep-Oct;57(5):447-8. doi: 10.1111/j.1550-7408.2010.00502.x.
Pneumocandins inhibit beta-1,3-glucan synthesis preventing the development of Pneumocystis cysts that are absent from the lungs of treated rats. To determine whether treated trophozoites are capable of DNA replication, cytochemical analyses were performed on 4',6-diamidino-2-phenylindole (DAPI)- and DB181-stained Pneumocystis carinii isolated from pneumocandin L-693-989-treated rats. Fluorescence intensities of trophozoite nuclei from drug-treated rats were greater than those of untreated controls, suggesting that DNA replication was not inhibited but that cytokinesis and perhaps karyokinesis were blocked.
肺囊菌素抑制β-1,3-葡聚糖合成，防止无肺孢子虫囊从接受治疗的大鼠肺部发育。为了确定受治疗的滋养体是否能够进行 DNA 复制，对从接受肺囊菌素 L-693-989 治疗的大鼠中分离出的 4',6-二脒基-2-苯吲哚（DAPI）和 DB181 染色的卡氏肺孢子虫进行了细胞化学分析。来自药物处理大鼠的滋养体核的荧光强度大于未处理对照物，这表明 DNA 复制未被抑制，但细胞分裂和可能的核分裂被阻断。
