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在高碳酸血症暴露的鱼类中测量细胞内 pH 值的验证：组织储存时间的影响和代谢抑制剂组织匀浆法的效果。

A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.

机构信息

Department of Zoology, 6270 University Blvd, University of British Columbia, Vancouver, BC V6T1Z4 Canada.

出版信息

J Fish Biol. 2009 Jul;75(1):268-75. doi: 10.1111/j.1095-8649.2009.02261.x.

DOI:10.1111/j.1095-8649.2009.02261.x
PMID:20738495
Abstract

This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).

摘要

本研究评估了组织储存时间和代谢抑制剂组织匀浆（MITH）方法的准确性对过度碳酸血症期间白鲟 Acipenser transmontanus 组织细胞内 pH 值（pHi）的影响。在液氮中储存长达 30 天没有观察到影响，并且 MITH 似乎适合测量暴露于高达 10% CO2（10000 Pa pCO2）的鱼类的 pH 值。

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