Dawson R, Phillips M I
Department of Pharmacodynamics, University of Florida, Gainesville 32610.
Eur J Pharmacol. 1990 Dec 15;189(6):423-6. doi: 10.1016/0922-4106(90)90041-u.
A porcine-derived renal epithelial cell line, LLC-PK1, was used to examine dopamine (DA) synthesis from L-dopa and DA release into the media. DA synthesis and release were elevated by increasing either L-dopa concentration or incubation time. DA synthesis was shown to be entirely due to conversion of L-dopa to DA by aromatic amino acid decarboxylase. DA release from LLC-PK1 cells was stimulated by NaCl and other Na+ or Cl- salts. DA release by LLC-PK1 cells was not dependent on extracellular Ca2+ or significantly stimulated by the depolarizing actions of KCl. LLC-PK1 cells which are devoid of any neural contributions to renal DA production can synthesize DA from L-dopa and release DA in response to stimulation by either Na+ or Cl-.
一种源自猪的肾上皮细胞系LLC-PK1，被用于检测左旋多巴（L-dopa）合成多巴胺（DA）以及DA释放到培养基中的情况。通过增加L-dopa浓度或孵育时间，DA的合成和释放均有所升高。DA的合成被证明完全是由于芳香族氨基酸脱羧酶将L-dopa转化为DA所致。LLC-PK1细胞释放DA受到NaCl以及其他Na⁺或Cl⁻盐的刺激。LLC-PK1细胞释放DA不依赖于细胞外Ca²⁺，也未被KCl的去极化作用显著刺激。LLC-PK1细胞对肾脏DA的产生没有任何神经方面的贡献，它可以从L-dopa合成DA，并在受到Na⁺或Cl⁻刺激时释放DA。
