Sandin Karin, Kloo Lars, Odselius Rolf, Olsson Lars-Fride
Gambro Corporate Research, Lund and Department of Chemistry, Royal Institute of Technology, Stockholm - Sweden.
J Appl Biomater Biomech. 2009 Jan-Apr;7(1):13-22.
Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca5(PO4)3(HCO3).4H2O with an estimated solubility constant of 6.10-39 (mol/L)9. Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity.
目的。血管中的磷酸钙沉积与死亡风险增加相关。在本研究中，对模拟血清无机部分的体外溶液中固体磷酸钙的形成进行了研究。方法。使用多种实验技术对形成的沉淀物进行分析，包括红外光谱、拉曼光谱、X射线能量色散光谱、燃烧气体化学分析、热重分析以及透射电子显微镜和扫描电子显微镜。结果。结果表明形成了一种微观上无定形但纳米晶的材料，具有整体磷灰石结构。确定了一个合理的化学计量式为Ca5(PO4)3(HCO3).4H2O，估计溶解度常数为6.10 - 39 (mol/L)9。水溶液中的碳酸氢盐被证明对沉淀至关重要，这对体外研究具有启示意义。结论。本研究中形成的磷酸钙在组成、形态和结晶度方面与病理性磷酸钙有许多相似之处。
