简化毛细管电泳纳米喷雾鞘流接口，用于提高肽分析的效率和灵敏度。

Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis.

机构信息

Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA.

出版信息

Rapid Commun Mass Spectrom. 2010 Sep 15;24(17):2554-60. doi: 10.1002/rcm.4672.

PMID:20740530

Abstract

We report a simple nanospray sheath-flow interface for capillary electrophoresis. This interface relies on electrokinetic flow to drive both the separation and the electrospray; no mechanical pump is used for the sheath flow. This system was interfaced with an LCQ mass spectrometer. The best results were observed with a 2-microm diameter emitter tip and a 1-mm spacing between the separation capillary tip and the emitter tip. Under these conditions, mass detection limits (3sigma) of 100 amol were obtained for insulin receptor fragment 1142-1153. The separation efficiency exceeded 200,000 plates for this compound. The relative standard deviation generated during continual infusion of a 50 microM solution of angiotensin II was 2% for the total ion count and 3% for the extracted ion count over a 40-min period. Finally, the interface was also demonstrated for negative ion mode.

摘要

我们报告了一种用于毛细管电泳的简单纳米喷雾鞘流接口。该接口依赖电动流来驱动分离和电喷雾；不需要机械泵来提供鞘流。该系统与 LCQ 质谱仪相连接。在使用 2 微米直径的喷嘴和分离毛细管尖端与喷嘴之间 1 毫米的间隔的情况下，观察到最佳结果。在这些条件下，获得了胰岛素受体片段 1142-1153 的 100 amol 的质量检测限（3sigma）。对于该化合物，分离效率超过 200,000 板。在 40 分钟的时间内，连续注入 50 microM 血管紧张素 II 溶液期间产生的相对标准偏差为总离子计数的 2%，提取离子计数的 3%。最后，还证明了该接口也适用于负离子模式。