Kele Zoltán, Ferenc Györgyi, Klement Eva, Tóth Gábor K, Janáky Tamás
Department of Medical Chemistry, University of Szeged, 6720 Szeged, Dóm tér 8, Hungary.
Rapid Commun Mass Spectrom. 2005;19(7):881-5. doi: 10.1002/rcm.1866.
A simple sheathless capillary electrophoresis (CE)/mass spectrometry (MS) interface was constructed by combining widely used nanospray needles with fused-silica capillaries and it was successfully applied for the separation of peptides. The end of the CE capillary was pulled to a taper, etched and then fitted into the metal-coated nanospray borosilicate capillary. The nanospray needle can be used for several CE runs, but it can be easily and rapidly changed in the case of accidental breakage or evaporation of the coating. A fast capillary electrochromatographic method was also developed for MS analysis of peptides containing numerous basic amino acids.
通过将广泛使用的纳米喷雾针与熔融石英毛细管相结合，构建了一种简单的无鞘毛细管电泳（CE）/质谱（MS）接口，并成功应用于肽的分离。将CE毛细管的末端拉成锥形，进行蚀刻，然后安装到涂有金属的纳米喷雾硼硅酸盐毛细管中。纳米喷雾针可用于多次CE运行，但在涂层意外破损或蒸发的情况下，可以轻松快速地更换。还开发了一种快速毛细管电色谱方法，用于对含有大量碱性氨基酸的肽进行MS分析。
