Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, USA.
J Biomed Mater Res A. 2010 Oct;95(1):40-8. doi: 10.1002/jbm.a.32815.
The in vitro adsorption of plasma proteins on polyanhydride microparticles based on sebacic acid (SA), 1,6-bis(p-carboxyphenoxy)hexane (CPH), and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) was studied. Three model proteins from bovine serum (albumin (BSA), immunoglobulin G (IgG), and fibrinogen (Fg)) were used. The adsorption was studied using X-Ray Photoelectron Spectroscopy and gel electrophoresis. 2D electrophoresis was used to study the adsorption of plasma proteins from bovine serum. Differences in the amount of protein adsorbed were detected as a function of the following: (i) copolymer composition and (ii) specific protein studied. A direct correlation between polymer hydrophobicity and protein adsorbed was observed and higher quantities of Fg and IgG were absorbed. In vitro release studies were performed with ovalbumin-encapsulated microparticles that were incubated with Fg; these studies showed a reduction in the amount of ovalbumin released from the microparticles when Fg is adsorbed on the surface. An understanding of protein adsorption patterns on parenteral delivery devices is valuable in optimizing their in vivo performance.
研究了基于癸二酸（SA）、1,6-双（对羧基苯氧基）己烷（CPH）和 1,8-双（对羧基苯氧基）-3,6-二氧辛烷（CPTEG）的聚酸酐微球对血浆蛋白的体外吸附。使用了三种来自牛血清的模型蛋白（白蛋白（BSA）、免疫球蛋白 G（IgG）和纤维蛋白原（Fg））。使用 X 射线光电子能谱法和凝胶电泳法研究了吸附作用。二维电泳用于研究牛血清中血浆蛋白的吸附情况。作为以下方面的函数，检测到吸附蛋白的量的差异：（i）共聚物组成和（ii）研究的特定蛋白。观察到聚合物疏水性与吸附蛋白之间存在直接相关性，并且 Fg 和 IgG 的吸附量更高。用纤维蛋白原孵育包封卵清蛋白的微球进行了体外释放研究；这些研究表明，当 Fg 吸附在表面上时，从微球中释放出的卵清蛋白的量减少。了解蛋白质在注射给药装置上的吸附模式对于优化其体内性能很有价值。
