Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
J Biomed Mater Res A. 2010 Oct;95(1):49-57. doi: 10.1002/jbm.a.32816.
Numerous in vitro studies have indicated the potential of using electrospun nanofibrous scaffolds for tissue regeneration. However, few reports have demonstrated their utility in real tissue repair models. The present investigation tested the hypothesis that electrospun poly-L-lactic acid (PLLA) nanofibrous membrane leads to dense cortical bone regeneration and improves the efficacy of currently-used collagenous guided bone regeneration (GBR) membrane. In vitro, the function of bone marrow-derived mesenchymal stem cells (BMSCs) on nanofibrous scaffolds was evaluated. In an in vivo experiment, large bony defects were created in rabbit tibia and treated with a nanofiber-reinforced bilayer membrane, nanofibrous membrane, or collagenous membrane alone. Three and six weeks after operation, bone defect healing was assessed radiologically and histologically. In vitro differentiation studies showed that BMSCs had much higher expression of Runx2 and collagen type I, alpha 1 mRNAs, when cultured on nanofibrous scaffolds. The radiographic and histological data both showed that the group treated with bilayer membrane had more bony tissue formation at 3 weeks. Moreover, at 6 weeks, only the bilayer membrane-treated bone defects displayed better regeneration of cortical bone tissue, whereas in the other groups the defects were filled with spongy bone-like tissue. The results demonstrated that electrospun nanofibrous membrane improves the regeneration of cortical bone, suggesting that this type of membrane can be combined with current collagenous GBR membrane to improve guided bone regeneration technology.
大量的体外研究表明，静电纺丝纳米纤维支架在组织再生方面具有潜力。然而，很少有报道证明它们在实际的组织修复模型中的有效性。本研究检验了以下假设：静电纺丝聚 L-乳酸（PLLA）纳米纤维膜可促进致密皮质骨再生，并提高目前使用的胶原引导骨再生（GBR）膜的效果。在体外，评估了骨髓间充质干细胞（BMSCs）在纳米纤维支架上的功能。在体内实验中，在兔胫骨上制造大的骨缺损，并单独用纳米纤维增强双层膜、纳米纤维膜或胶原膜进行处理。术后 3 周和 6 周时，通过影像学和组织学评估骨缺损的愈合情况。体外分化研究表明，当 BMSCs 在纳米纤维支架上培养时，其 Runx2 和胶原 I，alpha 1 mRNA 的表达水平更高。影像学和组织学数据均表明，在 3 周时，用双层膜处理的组形成了更多的骨组织。此外，在 6 周时，只有用双层膜处理的骨缺损显示出更好的皮质骨组织再生，而在其他组中，缺损填充了海绵骨样组织。结果表明，静电纺丝纳米纤维膜可促进皮质骨的再生，这表明这种膜可以与现有的胶原 GBR 膜结合，以改善引导骨再生技术。
