Zhang Chun-Lei, Tong Guang-Xiang, Kuang You-Yi, Zhang Chao, Yin Jia-Sheng
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China.
Dongwuxue Yanjiu. 2010 Aug;31(4):395-400. doi: 10.3724/SP.J.1141.2010.04395.
Using seven pairs of microsatellite markers we studied the relationship between the size of the the candidate groups and the microsatellite paternity testing accuracy by separate breed or the microsatellite paternity appraisal ability by mixed breed. By computer software analysis, the appraisal ability decreased with the candidate groups increase. For the 81 putative parents the microsatellite paternity testing accuracy was 80% and the microsatellite paternity appraisal ability was 78.9%; for the 9 putative parents, the testing accuracy was 93.3% and the appraisal ability was 92.2%. The results indicated that the microsatellite DNA markers can be used for the parentage determination of Hucho taimen Pallas.
我们使用7对微卫星标记，通过按品种分类研究候选群体大小与微卫星亲子鉴定准确性之间的关系，或通过混合品种研究微卫星亲权鉴定能力。通过计算机软件分析，随着候选群体数量增加，鉴定能力下降。对于81个推定亲本，微卫星亲子鉴定准确性为80%，微卫星亲权鉴定能力为78.9%；对于9个推定亲本，检测准确性为93.3%，鉴定能力为92.2%。结果表明，微卫星DNA标记可用于哲罗鲑的亲权鉴定。
