基于微卫星多态性的犬类亲子鉴定

Canine parentage testing based on microsatellite polymorphisms.

作者信息

Ichikawa Y, Takagi K, Tsumagari S, Ishihama K, Morita M, Kanemaki M, Takeishi M, Takahashi H

机构信息

Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.

出版信息

J Vet Med Sci. 2001 Nov;63(11):1209-13. doi: 10.1292/jvms.63.1209.

DOI:10.1292/jvms.63.1209

PMID:11767055

Abstract

To establish an accurate method for parentage testing in dogs, microsatellite DNA repeat length polymorphisms were examined. We selected twenty microsatellite markers reported previously and examined their application for parentage testing in Beagles and Labrador Retrievers. Heterozygosity (He), Polymorphism Information Content (PIC), the probabilities of Paternity Exclusion (PE) and the combined PE were calculated from allelic frequencies of the markers. All markers amplified by polymerase chain reactions were polymorphic and many markers showed high He and PIC in the both breeds. The final combined PEs in Beagles and Labrador Retrievers were 0.999994 and 0.999920, respectively. The results suggest that the twenty markers can be applied for routine parentage testing in dogs.

摘要

为建立一种准确的犬亲子鉴定方法，对微卫星DNA重复长度多态性进行了研究。我们选取了先前报道的20个微卫星标记，并检测了它们在比格犬和拉布拉多犬亲子鉴定中的应用。根据标记的等位基因频率计算杂合度（He）、多态信息含量（PIC）、排除父权概率（PE）及累积排除父权概率。通过聚合酶链反应扩增的所有标记均具有多态性，且许多标记在两个品种中均显示出高He和PIC。比格犬和拉布拉多犬的最终累积排除父权概率分别为0.999994和0.999920。结果表明，这20个标记可用于犬的常规亲子鉴定。