Parra G, Mosquera J, Rodríguez-Iturbe B
Renal Service and Laboratory, Hospital Universitario de Maracaibo, Venezuela.
Kidney Int. 1990 Dec;38(6):1118-24. doi: 10.1038/ki.1990.321.
Monocytes have been demonstrated to play an important role in acute serum sickness (AcSS) nephritis. Because accumulation of monocytes within the glomeruli could be the result of local lymphokine production, we studied migration inhibition factor (MIF) activity in supernatants from glomerular cultures, analyzed its temporal relationship with monocyte and lymphocyte accumulation, and tested the effect of anti-T lymphocyte monoclonal antibody on local MIF production. AcSS was induced in 12 rabbits, and one additional rabbit had antigen elimination without proteinuria. Single nephrectomy was performed at the time of antigen elimination in all animals; the remaining kidney was removed four days (4 rabbits) or 14 days afterwards (5 rabbits). In glomerular cross sections (gcs), lymphocytes were identified using monoclonal antibody M108, and monocytes by nonspecific esterase stain (ES). MIF activity was determined in supernatants of cultures of isolated glomeruli by the agarose microdroplet method. Peak of MIF activity (84.3 +/- 2.6%, SEM) was observed the first day of proteinuria in association with peak of lymphocyte infiltration (1.15 +/- 0.1 lymphocytes/gcs) and monocyte infiltration (2.4 +/- 0.3 mean ES score/gcs). MIF activity diminished by day 4 (66.0 +/- 6.3%) and reached control levels by day 14 (12.8 +/- 3.2%). There was a significant correlation between lymphocyte infiltration and MIF activity (r = 0.776, P less than 0.0001) as well as between MIF activity and monocyte accumulation (r = 0.858, P less than 0.0001). In five additional rabbits with AcSS, glomeruli were isolated, treated successively with M108 and normal rabbit serum, and supernatants harvested from 24-hour cultures were tested for MIF activity.(ABSTRACT TRUNCATED AT 250 WORDS)
单核细胞已被证明在急性血清病（AcSS）肾炎中起重要作用。由于肾小球内单核细胞的积聚可能是局部淋巴因子产生的结果，我们研究了肾小球培养上清液中的迁移抑制因子（MIF）活性，分析了其与单核细胞和淋巴细胞积聚的时间关系，并测试了抗T淋巴细胞单克隆抗体对局部MIF产生的影响。对12只兔子诱导AcSS，另外1只兔子进行抗原清除且无蛋白尿。所有动物在抗原清除时进行单侧肾切除术；4只兔子在4天后，5只兔子在14天后切除剩余肾脏。在肾小球横断面（gcs）中，使用单克隆抗体M108鉴定淋巴细胞，通过非特异性酯酶染色（ES）鉴定单核细胞。采用琼脂糖微滴法测定分离肾小球培养上清液中的MIF活性。在蛋白尿出现的第一天观察到MIF活性峰值（84.3±2.6%，标准误），同时伴有淋巴细胞浸润峰值（1.15±0.1个淋巴细胞/gcs）和单核细胞浸润峰值（平均ES评分2.4±0.3/gcs）。MIF活性在第4天降低（66.0±6.3%），到第14天达到对照水平（12.8±3.2%）。淋巴细胞浸润与MIF活性之间存在显著相关性（r = 0.776，P < 0.0001），MIF活性与单核细胞积聚之间也存在显著相关性（r = 0.858，P < 0.0001）。在另外5只患有AcSS的兔子中，分离肾小球，先后用M108和正常兔血清处理，对24小时培养收获的上清液检测MIF活性。（摘要截短至250字）
