Zakarija M, McKenzie J M
Endocr Res Commun. 1977;4(6):343-55. doi: 10.3109/07435807709073627.
The thyroid gland of rats fed propylthiouracil is known to be unresponsive in vitro to thyrotropin; to investigate further the underlying mechanism groups of rats were variously treated with propylthiouracil and thyroid hormone or subjected to hypophysectomy. In vitro responsiveness of the thyroids was tested by measuring an increase in the concentration of c AMP when thyrotropin or prostaglandin E1 was added to the medium. Results showed that responsiveness to thyrotropin partially returned with rats fed prophylthiouracil and hypophysectomized 5, but not 2, days before death; hypophysectomy of normal rats led to increased in vitro responsiveness to thyrotropin and this was partially reversed by injections of thyrotropin for a week before death. Administration of thyroid hormone had little effect in these investigations and in vitro responsiveness to prostaglanding E1 was not consistently influenced by any of the in vivo regimens. From this experience we conclude that, at least as studied in vitro, circulating thyrotropin has a significant role in modulating responsiveness of the thyroid to thyrotropin.
已知喂食丙硫氧嘧啶的大鼠甲状腺在体外对促甲状腺激素无反应；为了进一步研究其潜在机制，将大鼠分组，分别用丙硫氧嘧啶和甲状腺激素进行不同处理，或进行垂体切除。通过测量向培养基中添加促甲状腺激素或前列腺素E1时环磷酸腺苷（c AMP）浓度的增加来测试甲状腺的体外反应性。结果表明，喂食丙硫氧嘧啶并在死亡前5天而非2天进行垂体切除的大鼠，其对促甲状腺激素的反应性部分恢复；正常大鼠垂体切除导致体外对促甲状腺激素的反应性增加，而在死亡前一周注射促甲状腺激素可使其部分逆转。在这些研究中，甲状腺激素的给药几乎没有影响，并且体内任何一种处理方式对体外对前列腺素E1的反应性均无一致影响。从这些实验中我们得出结论，至少在体外研究中，循环中的促甲状腺激素在调节甲状腺对促甲状腺激素的反应性方面具有重要作用。
