一种用于未麻醉、未束缚大鼠的胆道和血管通路模型。

A model for biliary and vascular access in the unanesthetized, unrestrained rat.

作者信息

Epstein R E, Branum G D, Cucchiaro G, Meyers W C

机构信息

Department of Surgery, Duke University Medical Center, Durham, NC 27710.

出版信息

Physiol Behav. 1990 Oct;48(4):539-42. doi: 10.1016/0031-9384(90)90296-g.

DOI:10.1016/0031-9384(90)90296-g

PMID:2075205

Abstract

Conventional methods for vascular access and bile collection in the rat, such as the isolated perfused liver, study under anesthesia, and animal restraint, do not allow study of a physiologically intact rat. A simple technique for vascular access and monitoring of biliary secretions in the intact, unrestrained, unanesthetized rat for extended periods of time is described. Sample experiments demonstrated a 20% complication rate and an 85% 24-hour survival. This model may prove useful in studies requiring chronic vascular and visceral catheterization in the awake rat.

摘要

大鼠血管通路和胆汁采集的传统方法，如离体灌注肝脏、麻醉状态下研究和动物束缚，无法对生理状态完整的大鼠进行研究。本文描述了一种在完整、无束缚、未麻醉的大鼠中长时间进行血管通路建立和胆汁分泌监测的简单技术。样本实验显示并发症发生率为20%，24小时存活率为85%。该模型可能在需要对清醒大鼠进行慢性血管和内脏插管的研究中证明有用。