Rolf L L, Bartels K E, Nelson E C, Berlin K D
Department of Pathology, Oklahoma University Health Sciences Center, Oklahoma City.
Lab Anim Sci. 1991 Oct;41(5):486-92.
To our knowledge this is the first report of rat bile duct cannulations in which the distal cannula is hemisected but extends to the sphincter of Oddi. It is minimally invasive and requires only about 45 minutes preparation time. In contrast to studies described in the literature, enterohepatic recirculation remains intact but bile can always be separated from pancreatic secretions at investigator discretion in the model. In addition, biliary flow and pressure can be measured without compromise. Acute biliary secretory pressure, under anesthesia, was 17 cm water. Bile flow, averaging 9.6 microliters/min/100 g was measured in unanesthetized rats surviving for 2 weeks (60% of animals monitored). Gross necropsy findings indicated that animals dying in less than 7 days usually suffered bile peritonitis subsequent to catheter rupture of the bile duct or loss from the ligature restraint. Deaths after 2 weeks were usually related to cholestasis due to blockage of the catheter with mineral debris and/or duct tissue. A detailed literature review of bile duct cannulation in rats has been made.
据我们所知，这是首次关于大鼠胆管插管的报告，其中远端插管被对半切开但延伸至Oddi括约肌。该方法微创，仅需约45分钟的准备时间。与文献中描述的研究不同，在该模型中肝肠循环保持完整，但研究者可根据需要随时将胆汁与胰液分离。此外，可在不影响其他指标的情况下测量胆汁流量和压力。麻醉状态下急性胆汁分泌压力为17厘米水柱。在存活2周的未麻醉大鼠（监测动物的60%）中测得胆汁流量平均为9.6微升/分钟/100克。大体尸检结果表明，在7天内死亡的动物通常在胆管导管破裂或结扎处胆汁漏出后发生胆汁性腹膜炎。2周后死亡通常与导管被矿物质碎片和/或导管组织堵塞导致的胆汁淤积有关。已对大鼠胆管插管进行了详细的文献综述。
