Lee J V, Akunne H C, Soliman K F
College of Pharmacy, Florida A & M University, Tallahassee 32307.
Arch Int Pharmacodyn Ther. 1990 Jul-Aug;306:34-44.
In this study, male Sprague-Dawley rats (150-230 g), maintained under controlled lighting and temperature conditions, were used. In one experiment, glucose administration (10 g/kg) was found to be associated with profound analgesia which could be blocked by prior administration of atropine (0.5 mg/kg). In another experiment, when two doses of glucose were given at 24 hr interval, the second injection of glucose was associated with tolerance to glucose-induced analgesia. In an attempt to correlate changes in the cholinergic enzymes with glucose-induced analgesia, choline acetyltransferase and acetylcholinesterase activities were determined in the cerebral cortex, bulbus olfactorius, midbrain, hypothalamus, hippocampus, cerebellum, pons and medulla oblongata in control rats and rats treated with a single dose of glucose (10 g/kg) or two doses of glucose. The second administration of glucose was accompanied with tolerance in the level of acetylcholinesterase in the bulbus olfactorius, midbrain, cerebral cortex, cerebellum and pons. There were no significant changes in choline acetyltransferase activities between the groups studied. The results obtained indicate that the cholinergic system may be involved in glucose-induced analgesia and that the rapid development of tolerance to glucose-induced analgesia is associated with the tolerance in the response of brain acetylcholinesterase activity.
在本研究中，使用了体重150 - 230克、饲养于光照和温度受控条件下的雄性斯普拉格-道利大鼠。在一项实验中，发现给予葡萄糖（10克/千克）会产生深度镇痛作用，而预先给予阿托品（0.5毫克/千克）可阻断这种作用。在另一项实验中，当每隔24小时给予两剂葡萄糖时，第二次注射葡萄糖会导致对葡萄糖诱导的镇痛产生耐受性。为了将胆碱能酶的变化与葡萄糖诱导的镇痛作用相关联，测定了对照组大鼠以及接受单剂量葡萄糖（10克/千克）或两剂量葡萄糖处理的大鼠大脑皮质、嗅球、中脑、下丘脑、海马体、小脑、脑桥和延髓中的胆碱乙酰转移酶和乙酰胆碱酯酶活性。第二次给予葡萄糖伴随着嗅球、中脑、大脑皮质、小脑和脑桥中乙酰胆碱酯酶水平的耐受性。所研究的各组之间胆碱乙酰转移酶活性没有显著变化。获得的结果表明，胆碱能系统可能参与葡萄糖诱导的镇痛作用，并且对葡萄糖诱导的镇痛作用耐受性的快速发展与脑乙酰胆碱酯酶活性反应的耐受性有关。
