Minder C, Vanden Driessche T
Differentiation. 1978 May 26;10(3):165-70. doi: 10.1111/j.1432-0436.1978.tb00959.x.
The 3',5'-adenosine monophosphate (cyclic-AMP) content of the unicellular alga Acetabularia has been examined at various developmental stages. It has been found that very young algae, less than 10mm in length, have a high cAMP content [more than 7 pmoles per 100 mg wet weight (WW)], but that with the growth of the algae, the cAMP content decreases rapidly, reaching the low level of 0.5--1.0 pmoles per 100mg WW. The cAMP content remains at this level until cap differentiation, after which an increase in cAMP content accompanies cap enlargement. It has been shown that these results are unlikely to be affected by changes in the cAMP content induced by variations in circadian rhythm. Treatment with theophylline (2.10(-3) M), a phosphodieterase inhibitor, results in an increase in the cAMP content and delays growth and cap formation. Experiments on the effects of theophylline upon the circadian rhythm of oxygen evolution have shown that the continuous presence of theophylline in the culture medium does not induce a phase shift in the rhythm. The cAMP content of anucleate Acetabularia shows development stage variations parallel to that of the whole algae.
已在单细胞藻类伞藻的不同发育阶段检测了3',5'-腺苷单磷酸（环磷酸腺苷，cAMP）的含量。研究发现，长度小于10毫米的非常幼嫩的藻类，其cAMP含量较高[每100毫克湿重（WW）超过7皮摩尔]，但随着藻类生长，cAMP含量迅速下降，降至每100毫克WW 0.5 - 1.0皮摩尔的低水平。cAMP含量在帽状体分化之前一直保持在这个水平，帽状体分化后，随着帽状体增大，cAMP含量增加。研究表明，这些结果不太可能受到昼夜节律变化引起的cAMP含量变化的影响。用磷酸二酯酶抑制剂茶碱（2×10⁻³ M）处理会导致cAMP含量增加，并延迟生长和帽状体形成。关于茶碱对氧气释放昼夜节律影响的实验表明，培养基中持续存在茶碱不会引起节律的相位偏移。无核伞藻的cAMP含量显示出与整个藻类平行的发育阶段变化。
