Jensen M E, Wefel J S, Triolo P T, Hammesfahr P D
University of Iowa, Iowa City.
Am J Dent. 1990 Apr;3(2):75-8.
This study examined the effect of a fluoride-releasing fissure sealant upon enamel demineralization in an artificial-caries system. Twenty extracted human molars were randomly assigned to two treatment groups consisting of (1) conventional fissure sealant and (2) fluoride-releasing sealant. Specimens were acid-etched, rinsed, dried, sealant applied, and photocured for 20 seconds. Teeth were thermocycled 500x between 5 degrees C and 55 degrees C using 1 minute dwell times. Acid-resistant varnish was placed on the specimens to within 1 mm of the sealant and the teeth were subjected to an artificial-caries challenge at pH 4.2 for 62 hours. Teeth were then sectioned and the thin-sections photographed using a polarizing-light microscope. Areas of the artificial-caries lesions and their depths were measured using a sonic-digitizing pad. Mean lesion depths (in arbitrary units from the digitizing pads) were 4.17 +/- 0.95 for Group 1 and 1.22 +/- 0.61 for Group 2. Mean areas were 17.26 +/- 7.32 for Group 1 and 3.12 +/- 2.59 for Group 2. Students' t-tests indicated statistically significant differences (P less than 0.001) between groups for both measures. This artificial-caries study indicates that a fluoride-releasing fissure sealant substantially reduces the amount of enamel demineralization adjacent to the material.
本研究在人工龋系统中检测了含氟窝沟封闭剂对牙釉质脱矿的影响。将20颗拔除的人类磨牙随机分为两个治疗组，分别为：（1）传统窝沟封闭剂组和（2）含氟窝沟封闭剂组。对样本进行酸蚀、冲洗、干燥，涂抹封闭剂并光固化20秒。牙齿在5℃至55℃之间进行500次热循环，每次保持1分钟。在样本上距封闭剂1毫米以内的区域涂抹抗酸漆，然后将牙齿置于pH值为4.2的人工龋环境中62小时。之后将牙齿切片，使用偏光显微镜拍摄薄片。使用声波数字化仪测量人工龋损的面积及其深度。第一组的平均龋损深度（数字化仪的任意单位）为4.17±0.95，第二组为1.22±0.61。第一组的平均面积为17.26±7.32，第二组为3.12±2.59。学生t检验表明，两组在这两项测量指标上均存在统计学显著差异（P＜0.001）。这项人工龋研究表明，含氟窝沟封闭剂可显著减少材料附近牙釉质的脱矿量。
