Akasaka Y
Department of Pathology, School of Medicine, Keio University, Tokyo, Japan.
Hum Cell. 1990 Oct;3(3):193-200.
Bone marrow and spleen are the major hematopoietic tissue in adult mice. However, little is known about the specific mechanism regulating hematopoiesis within these tissues. Since Dexter et al. first described conditions to maintain bone marrow hematopoiesis, long term bone marrow culture (LTBMC) has been developed in order to analyze the mechanism of the maintenance of proliferation and differentiation of hematopoietic stem cells in vitro. Furthermore, several stromal cell lines which are able to support the growth and differentiation of hematopoietic lineage, has been established from LTBMC. Although it is well known that bone marrow stromal cell lines are able to produce colony stimulating factors, it has been suggested that the stromal cell factors which involve membrane bound moieties must have a key role in the regulation of hematopoiesis. We expect that monoclonal antibodies to the surface of bone marrow stromal cells could detect such a critical stroma-associated protein that bounds the cell surface of the bone marrow stroma.
骨髓和脾脏是成年小鼠的主要造血组织。然而，对于这些组织内调节造血作用的具体机制知之甚少。自从德克斯特等人首次描述维持骨髓造血作用的条件以来，为了在体外分析造血干细胞增殖和分化维持的机制，长期骨髓培养（LTBMC）得以发展。此外，已经从长期骨髓培养中建立了几种能够支持造血谱系生长和分化的基质细胞系。尽管众所周知骨髓基质细胞系能够产生集落刺激因子，但有人提出涉及膜结合部分的基质细胞因子在造血调节中必定起关键作用。我们期望针对骨髓基质细胞表面的单克隆抗体能够检测到这样一种与骨髓基质细胞表面结合的关键的基质相关蛋白。
