Recovery of octyl beta-glucoside from detergent/protein mixtures.
作者信息
Kobs S F
机构信息
Department of Chemistry, Ohio State University, Columbus 43210.
出版信息
Anal Biochem. 1990 Nov 15;191(1):47-9. doi: 10.1016/0003-2697(90)90385-m.
PMID:2077942
Abstract
Octyl beta-glucoside is a nonionic detergent which is useful in the purification and characterization of membrane proteins. However, the cost of this reagent is prohibitive for large-scale protein purifications. A simple procedure to recover pure octyl beta-glucoside from mixtures of detergents with proteins and buffer salts is described.
摘要
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