Ashorn P, Moss B, Berger E A
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Ann N Y Acad Sci. 1990;616:149-54. doi: 10.1111/j.1749-6632.1990.tb17835.x.
CD4(178)-PE40 is a recombinant protein consisting of the HIV envelope glycoprotein-binding region of human CD4 linked to active domains of Pseudomonas aeruginosa exotoxin A. The hybrid toxin selectively kills HIV-infected human T-cell lines and protects against HIV spread in mixtures of uninfected and infected cells. We now report that CD4(178)-PE40 also selectively kills chronically HIV-1-infected cells of monocyte/macrophage lineage. The results provide further support for therapeutic use of this hybrid toxin in the treatment of HIV-infected individuals.
CD4(178)-PE40是一种重组蛋白，由与铜绿假单胞菌外毒素A的活性结构域相连的人CD4的HIV包膜糖蛋白结合区域组成。这种杂交毒素能选择性杀死感染HIV的人T细胞系，并防止HIV在未感染和已感染细胞的混合物中传播。我们现在报告，CD4(178)-PE40还能选择性杀死单核细胞/巨噬细胞系的慢性HIV-1感染细胞。这些结果为这种杂交毒素在治疗HIV感染个体中的治疗应用提供了进一步的支持。
