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[使用合成树脂包埋标本进行肾小管系统的扫描电子显微镜研究]

[Use of synthetic resin cases for the scanning electron microscopic study of the kidney tubule system].

作者信息

Magaudda L, Cutroneo G, De Leo S, Pisani A, Santoro G, Anastasi G

机构信息

Dipartimento di Biomorfologia, Università di Messina.

出版信息

Arch Ital Anat Embriol. 1990 Apr-Jun;95(2):87-104.

PMID:2078095
Abstract

Aim of our present work was to investigate a new method to study the three-dimensional arrangement, the length and the diameter of the different parts of the renal tubules. The ureter was cannulated after blocking the urinary flow with a binding of the ureter itself at its intermediate third, and injected in it against flow a synthetic resin (Mercox) normally used for vascular corrosion casts. It was demonstrated that the binding maintained only for 24 hours is adequate for morphological studies of the urinary tracts from papillar ducts until the Henle's loop. On the contrary the binding maintained for 7 days induced marked changes in the tubular architecture similar to the first anatomo-pathological changes of the nephrosclerosis following a chronic obstructive nephropathy.

摘要

我们当前工作的目的是研究一种新方法,以探究肾小管不同部分的三维排列、长度和直径。在输尿管中间三分之一处将其自身结扎以阻断尿流后,对输尿管进行插管,并逆向注入一种通常用于血管铸型的合成树脂(Mercox)。结果表明,仅维持24小时的结扎对于从乳头管到髓袢的尿路形态学研究就足够了。相反,维持7天的结扎会导致肾小管结构发生明显变化,类似于慢性梗阻性肾病后肾硬化的最初解剖病理学变化。

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