一种针对与人尿脱氧核糖核酸酶I N端氨基酸序列对应的合成肽的特异性抗体在人脱氧核糖核酸酶I同工酶基因分析中的实际应用。
Practical utility of an antibody specific to a synthetic peptide corresponding to the N-terminal amino acid sequence of human urine DNAse I for genetic analysis of human DNase I isozymes.
作者信息
Yasuda T, Nadano D, Tanaka Y, Sato W, Nakanaga M, Kishi K
机构信息
Department of Legal Medicine, Fukui Medical School, Japan.
出版信息
Biochem Int. 1990 Nov;22(4):699-705.
An antibody specific to a synthetic peptide corresponding to the N-terminal 27 amino acid residues of human urine DNase I (anti-DNase I peptide) was obtained. The antibody did not inhibit the activity of the enzyme, but reacted well with the enzyme upon immunoblotting following electrophoresis. The urine DNase I isozyme patterns detected using this antibody were almost identical to those produced with an antibody specific to purified DNase I. Therefore, the anti-DNase I peptide antibody should prove to be valuable for genetic analysis of human DNase I isozymes.
获得了一种针对与人尿脱氧核糖核酸酶I（抗DNase I肽）N端27个氨基酸残基对应的合成肽的特异性抗体。该抗体不抑制酶的活性，但在电泳后的免疫印迹中与该酶反应良好。使用此抗体检测到的尿DNase I同工酶模式与使用针对纯化DNase I的特异性抗体产生的模式几乎相同。因此，抗DNase I肽抗体对于人类DNase I同工酶的遗传分析应具有重要价值。
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