Jeffrey I W, Kelly F J, Duncan R, Hershey J W, Pain V M
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.
Biochimie. 1990 Oct;72(10):751-7. doi: 10.1016/0300-9084(90)90160-i.
The ability of the initiation factor eIF-2 in skeletal muscle extracts to form ternary initiation complexes ([Met-tRNA(f).eIF-2.GDP]) is decreased by either starvation or diabetes. These conditions also impair the ability of muscle extracts to dissociate [eIF-2.GDP], suggesting inhibition of the guanine nucleotide exchange reaction essential for eIF-2 recycling. We could not, however, detect any change in the phosphorylation state of the alpha subunit of eIF-2. This suggests that eIF-2 activity may be regulated in this system by a mechanism not involving its phosphorylation.
