Volk H W, Schneider J, Dämmrich J, Döll W, Hörl M, Bruch H P
Chirurgische Universitätsklinik Würzburg, BRD.
Eur Surg Res. 1990;22(6):347-55. doi: 10.1159/000129121.
Clinical experience gives evidence of the increasing importance of chronic-abscess-forming peritonitis with a general physical impairment in spite of a well-controlled abdominal situation. The number of unsolved problems led us to develop a standardized and reproducible experimental animal model, using Wistar rats. We induced peritonitis by intraperitoneal injection of a mixture containing a capsulated strain of Bacteroides fragilis and DEV agar. The histologic changes in the parietal and visceral peritoneum, lymphnodes and liver were recorded in the course of the disease. The data largely correlate with those recorded in human peritonitis. Bacteriologic examinations gave evidence of bacteria passing through the intestinal wall into the abdomen. This is the first model for peritonitis which is defined by its histological and bacteriological course. It enables one to examine pathophysiological problems and the consequences of harm and stress for the course of the disease.
临床经验表明，尽管腹部情况得到良好控制，但慢性脓肿形成性腹膜炎伴全身身体功能损害的重要性日益增加。未解决问题的数量促使我们开发一种标准化且可重复的实验动物模型，使用Wistar大鼠。我们通过腹腔注射含有脆弱拟杆菌包膜菌株和DEV琼脂的混合物诱导腹膜炎。在疾病过程中记录了壁腹膜、脏腹膜、淋巴结和肝脏的组织学变化。这些数据与人类腹膜炎中记录的数据在很大程度上相关。细菌学检查证明细菌穿过肠壁进入腹腔。这是第一个根据其组织学和细菌学过程定义的腹膜炎模型。它使人们能够研究病理生理问题以及损伤和应激对疾病进程的影响。
