Hubens G, Willems G
Cancer Research Unit, Faculty of Medicine, Vrije Universiteit, Brussels, Belgium.
Eur Surg Res. 1990;22(6):356-64. doi: 10.1159/000129122.
An experimental model is proposed to study suture line recurrence. By giving enemas with K12 colon carcinoma cells, derived from a 1,2-dimethylhydrazine-induced colon cancer in male syngeneic BDIX rats, we were able to obtain a suture implantation in about 30% of the control animals. Possible systemic and local factors which might influence this implantation rate could be studied using this model. Only local irrigations of the colon lumen with sodium hypochlorite 0.2% prior to anastomosis produced a significant decrease in implantation rate. Irrigations with chlorhexidine-cetrimide 2.5%, the use of iodized suture material and altering the cellular immune system of the hosts by ciclosporin or excessive surgical stress, had no significant effect on the implantation rate.
提出了一种实验模型来研究缝合线复发情况。通过给雄性同基因BDIX大鼠经1,2 - 二甲基肼诱导的结肠癌所衍生的K12结肠癌细胞灌肠，我们能够在约30%的对照动物中实现缝合线植入。利用该模型可以研究可能影响这种植入率的全身和局部因素。仅在吻合术前用0.2%的次氯酸钠对结肠腔进行局部冲洗，可使植入率显著降低。用2.5%的氯己定 - 西曲溴铵冲洗、使用碘化缝合材料以及通过环孢素或过度手术应激改变宿主的细胞免疫系统，对植入率均无显著影响。
