Morris G R, Walmsley L M, Brodie R R, Chasseaud L F
Department of Metabolism and Pharmacokinetics, Huntingdon Research Centre Ltd., U.K.
J Chromatogr. 1990 Sep 14;530(2):377-85. doi: 10.1016/s0378-4347(00)82340-3.
A simple, sensitive and selective method for the determination of ximoprofen and its keto and hydroxy metabolites in human urine has been developed using high-performance liquid chromatography in the reversed-phase mode. The limit of reliable determination of ximoprofen and each of its metabolites in urine is about 1 microgram/ml (4 nmol/ml). The method has been applied to urine samples obtained from human volunteers after administration of single intravenous doses of 30 mg of ximoprofen and about 70% dose was accounted for in terms of these compounds and their glucuronic acid conjugates.
已开发出一种简单、灵敏且具选择性的方法，采用反相高效液相色谱法测定人尿中的希莫洛芬及其酮类和羟基代谢物。尿中希莫洛芬及其每种代谢物的可靠测定限约为1微克/毫升（4纳摩尔/毫升）。该方法已应用于单次静脉注射30毫克希莫洛芬后从人体志愿者获取的尿样，这些化合物及其葡萄糖醛酸共轭物约占给药剂量的70%。
