Koshiishi I, Kobori Y, Imanari T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
J Chromatogr. 1990 Oct 26;532(1):37-43. doi: 10.1016/s0378-4347(00)83749-4.
A high-performance liquid chromatographic method was developed for the determination of citrulline and homocitrulline using a post-column colorimetric reaction with o-phthaladehyde and N-(1-naphthyl)-ethylenediamine. Citrulline and homocitrulline were determined with no interferences from protein amino acids. The results show that the level of citrulline in the plasma of patients with uremia on intermittent hemodialysis is higher than that in healthy human plasma, and that homocitrulline is excreted into the urine of healthy adults.
建立了一种高效液相色谱法，用于测定瓜氨酸和高瓜氨酸，该方法采用邻苯二甲醛和N-（1-萘基）-乙二胺柱后比色反应。瓜氨酸和高瓜氨酸的测定不受蛋白质氨基酸的干扰。结果表明，间歇性血液透析的尿毒症患者血浆中瓜氨酸水平高于健康人血浆，且高瓜氨酸可排泄到健康成年人的尿液中。
