[Effect of testosterone in Ca2+ mobilization in oocytes and porcine granulose stimulated by prolactin and theophylline].

Influence of testosterone in Ca2+ exchange in oocytes and porcine granulose stimulated by prolactin and theophylline was investigated using fluorescent dye chlortetracycline. It was shown that prolactin and theophylline separately have not affected on the Ca2+ exit from intracellular stores of oocytes. Prolactin and theophylline in common stimulate exit of Ca2+ from intracellular stores in oocytes, which decreased at presence of inhibitor of protein kinase A. Ca2+ exit from intracellular stores of oocytes stimulated by prolactin and theophylline in common was inhibited by thapsigargin. In the presence of testosterone prolactin and theophylline separately stimulate increasing of cytoplasmic Ca2+ concentration and exit of Ca2+ from intracellular stores of granulose. Prolactin and theophylline in common activate additional exit of Ca2+ from intracellular stores in granulose, which decreased in presence of inhibitor of protein kinas C. The date obtained suggest differences in regulated mechanisms of cell signaling in porcine granulose and oocytes.