通过酚类成分预测特级初榨橄榄油品种。对人乳腺癌细胞的潜在细胞毒性活性。
Prediction of extra virgin olive oil varieties through their phenolic profile. Potential cytotoxic activity against human breast cancer cells.
机构信息
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Fuentenueva s/n, E 18071 Granada, Spain.
出版信息
J Agric Food Chem. 2010 Sep 22;58(18):9942-55. doi: 10.1021/jf101502q.
The aim of this work was to develop a rapid resolution liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (RRLC-ESI-TOF-MS) method followed by tetrazolium salt (MTT)-based cell viability assays for qualitative and quantitative classification of extra virgin olive oil (EVOO) varieties by phenolic and other polar compound contents as well as for rapid characterization of putative cytotoxic activities against human cancer cells. Five different Spanish EVOO varieties were analyzed, and RRLC-ESI-TOF-MS method was applied for qualitative and quantitative identification of most important phenolic compounds. We finally employed MTT-based cell viability protocol to assess the effects of crude EVOO phenolic extracts (PEs) on the metabolic status of cultured SKBR3 human breast cancer cells. MTT-based cell viability assays revealed a wide range of breast cancer cytotoxic potencies among individual crude PE obtained from EVOO monovarietals. Remarkably, breast cancer cell sensitivity to crude EVOO-PEs was up to 12 times higher in secoiridoids enriched-PE than in secoiridoids-low/null EVOO-PE.
本工作旨在建立一种快速分辨液相色谱-电喷雾电离飞行时间质谱（RRLC-ESI-TOF-MS）方法，随后采用四唑盐（MTT）基于细胞活力检测法，通过酚类和其他极性化合物的含量对特级初榨橄榄油（EVOO）品种进行定性和定量分类，并对潜在的抗人类癌细胞细胞毒性活性进行快速表征。分析了五种不同的西班牙特级初榨橄榄油，应用 RRLC-ESI-TOF-MS 方法定性和定量鉴定了最重要的酚类化合物。最后，我们采用 MTT 基于细胞活力的方案来评估粗制 EVOO 酚类提取物（PEs）对培养的 SKBR3 人乳腺癌细胞代谢状态的影响。MTT 基于细胞活力的检测法揭示了从单品种 EVOO 获得的各个粗制 PE 对乳腺癌细胞的细胞毒性活性存在广泛的差异。值得注意的是，富含次级胆汁酸的粗制 EVOO-PE 对乳腺癌细胞的敏感性比次级胆汁酸低/无 EVOO-PE 高 12 倍。
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