一种用于体外和体内纳米毒性研究的纳米颗粒分散方法。

A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study.

机构信息

Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA.

出版信息

Nanotoxicology. 2010 Mar;4(1):42-51. doi: 10.3109/17435390903374019.

PMID:20795901

Abstract

The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (approximately 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO(2), Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.

摘要

不同尺寸、材料和形态的纳米颗粒在空气中的分散，以及涉及气溶胶输送的受控团聚，这是纳米颗粒毒理学领域最具挑战性的难题之一。我们在这里描述了一种使用电喷雾方法的纳米颗粒分散系统，用于提供具有空间均匀性和可控制的颗粒浓度的空气传播纳米颗粒（约 10-100nm），以便进行体外和体内研究。通过该分散方法，可以通过空气将单分散纳米颗粒（聚苯乙烯乳胶颗粒、TiO(2)、Au、Mn、量子点和碳纳米管）递送到细胞和动物体内。通过改变悬浮液进料速率，可以控制团聚程度，以模拟暴露研究的实际条件。