Lacroix Ghislaine, Koch Wolfgang, Ritter Detlef, Gutleb Arno C, Larsen Søren Thor, Loret Thomas, Zanetti Filippo, Constant Samuel, Chortarea Savvina, Rothen-Rutishauser Barbara, Hiemstra Pieter S, Frejafon Emeric, Hubert Philippe, Gribaldo Laura, Kearns Peter, Aublant Jean-Marc, Diabaté Silvia, Weiss Carsten, de Groot Antoinette, Kooter Ingeborg
Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
In Vitro und Mechanistische Toxikologie, Fraunhofer ITEM, Hannover, Germany.
Appl In Vitro Toxicol. 2018 Jun 1;4(2):91-106. doi: 10.1089/aivt.2017.0034.
air-liquid interface (ALI) cell culture models can potentially be used to assess inhalation toxicology endpoints and are usually considered, in terms of relevancy, between classic (i.e., submerged) models and animal-based models. In some situations that need to be clearly defined, ALI methods may represent a complement or an alternative option to experimentations or classic methods. However, it is clear that many different approaches exist and that only very limited validation studies have been carried out to date. This means comparison of data from different methods is difficult and available methods are currently not suitable for use in regulatory assessments. This is despite inhalation toxicology being a priority area for many governmental organizations. In this setting, a 1-day workshop on ALI models for respiratory toxicology research was organized in Paris in March 2016 to assess the situation and to discuss what might be possible in terms of validation studies. The workshop was attended by major parties in Europe and brought together more than 60 representatives from various academic, commercial, and regulatory organizations. Following plenary, oral, and poster presentations, an expert panel was convened to lead a discussion on possible approaches to validation studies for ALI inhalation models. A series of recommendations were made and the outcomes of the workshop are reported.
气液界面（ALI）细胞培养模型有可能用于评估吸入毒理学终点，并且通常就相关性而言，被认为介于经典（即浸没式）模型和基于动物的模型之间。在一些需要明确界定的情况下，ALI方法可能是实验或经典方法的一种补充或替代选择。然而，很明显存在许多不同的方法，并且迄今为止仅进行了非常有限的验证研究。这意味着来自不同方法的数据比较困难，并且目前可用的方法不适合用于监管评估。尽管吸入毒理学是许多政府组织的优先领域，但情况依然如此。在此背景下，2016年3月在巴黎举办了一次关于用于呼吸毒理学研究的ALI模型的为期一天的研讨会，以评估现状并讨论在验证研究方面可能实现的目标。欧洲的主要团体参加了该研讨会，汇集了来自各学术、商业和监管组织的60多名代表。在全体会议、口头报告和海报展示之后，召集了一个专家小组来主导关于ALI吸入模型验证研究可能方法的讨论。提出了一系列建议，并报告了研讨会的成果。
