Yacoub L K, Vanni T M, Goldberg I J
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, NY 10032.
J Lipid Res. 1990 Oct;31(10):1845-52.
Combined lipase deficiency (cld) is a genetic abnormality in mice resulting in the production of enzymatically inactive lipoprotein lipase (LPL). After suckling, these mice have markedly elevated levels of circulating triglyceride. An alteration of LPL gene expression in cld mice may affect the amount and/or the distribution of LPL mRNA in different cell types. Therefore, we performed in situ hybridization for LPL mRNA in tissues from normal and cld pups and adult mice using an antisense 35S-labeled cRNA probe. LPL mRNA had the same pattern of distribution in both cld and normal newborn mice; the probe hybridized strongly to pyramidal neurons of the hippocampus, heart myocytes, and hepatocytes. Despite the lack of noticeable fat stores, LPL mRNA was found in the dermal layer of the skin of cld mice and normal littermates. In adult mice, the cRNA probe for LPL hybridized to the hippocampus, to the heart, and to localized areas of the kidney. We conclude that despite great variation in plasma triglyceride levels, LPL gene is similarly expressed in animals with or without LPL activity.
联合脂肪酶缺乏症（cld）是小鼠中的一种基因异常，导致产生无酶活性的脂蛋白脂肪酶（LPL）。哺乳后，这些小鼠循环甘油三酯水平显著升高。cld小鼠中LPL基因表达的改变可能会影响不同细胞类型中LPL mRNA的数量和/或分布。因此，我们使用反义35S标记的cRNA探针，对正常和cld幼崽及成年小鼠的组织进行了LPL mRNA的原位杂交。在cld和正常新生小鼠中，LPL mRNA具有相同的分布模式；探针与海马体的锥体神经元、心肌细胞和肝细胞强烈杂交。尽管缺乏明显的脂肪储存，但在cld小鼠和正常同窝小鼠的皮肤真皮层中均发现了LPL mRNA。在成年小鼠中，LPL的cRNA探针与海马体、心脏和肾脏的局部区域杂交。我们得出结论，尽管血浆甘油三酯水平差异很大，但LPL基因在有或没有LPL活性的动物中表达相似。
