Mochida S, Poulain B, Eisel U, Binz T, Kurazono H, Niemann H, Tauc L
Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
J Physiol (Paris). 1990;84(4):278-84.
mRNAs encoding the light chain of tetanus and botulinum neurotoxins were transcribed, in vitro, from the cloned and specifically truncated genes of Clostridium tetani and Clostridium botulinum, respectively, and injected into presynaptic identified cholinergic neurons of the buccal ganglia of Aplysia californica. The size of the current response measured in the voltage clamped postsynaptic neuron was taken as indicator of the quantity of acetylcholine released. Depression of neurotransmitter release similar to that observed when native light chains of the two toxins were injected but needing an additional delay of 30 to 40 minutes, demonstrated a successful expression of a foreign mRNA injected into a neuron in situ.
分别从破伤风梭菌和肉毒杆菌克隆并经过特定截短的基因，在体外转录出编码破伤风和肉毒杆菌神经毒素轻链的信使核糖核酸（mRNA），并将其注入加州海兔颊神经节中已确定的突触前胆碱能神经元。在电压钳制的突触后神经元中测量的电流反应大小，被用作乙酰胆碱释放量的指标。神经递质释放的抑制情况与注射两种毒素的天然轻链时观察到的相似，但需要额外延迟30到40分钟，这表明注入原位神经元的外源mRNA成功表达。
