Zhang Xiaoxin, Zhang Lei, Huo Shuying, Wang Jianlin, Cui Sheng
College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Reprod Fertil Dev. 2010;22(7):1148-58. doi: 10.1071/RD09271.
The ovarian sympathetic nerves participate in the regulation of mammalian ovarian function, but it is still not known whether the neonatal ovarian sympathetic nerve is involved in follicular development and related mechanisms. In the present study, the superior ovarian nerve (SON) of the neonatal rat was transected on postnatal day (PD) 2, and follicle development, ovarian hormone secretion, ovulation rate, granulosa cell proliferation and apoptosis were analysed on PD 30 and PD 90. The results demonstrate that SON transection decreases follicle number and size, reduces ovulation induced by gonadotrophin and enhances follicular atresia. Bromodeoxyuridine (BrdU) and cleaved caspase-3 immunohistochemistry staining provide evidence that SON transection inhibits granulosa cell proliferation and promotes granulosa cell apoptosis. In addition, SON transection increases serum oestradiol levels, but has no influence on serum progesterone levels. These results suggest that the sympathetic nerve supply to the ovaries is important in regulating follicle development and ovary function. These results are critical for further understanding of the neuroendocrine regulation of ovary development and function, although the mechanism needs to be elucidated in future studies.
卵巢交感神经参与哺乳动物卵巢功能的调节，但新生儿卵巢交感神经是否参与卵泡发育及其相关机制尚不清楚。在本研究中，在出生后第2天（PD2）切断新生大鼠的卵巢上神经（SON），并在PD30和PD90分析卵泡发育、卵巢激素分泌、排卵率、颗粒细胞增殖和凋亡情况。结果表明，切断SON会减少卵泡数量和大小，降低促性腺激素诱导的排卵，并增强卵泡闭锁。溴脱氧尿苷（BrdU）和裂解的半胱天冬酶-3免疫组化染色提供了证据，表明切断SON会抑制颗粒细胞增殖并促进颗粒细胞凋亡。此外，切断SON会增加血清雌二醇水平，但对血清孕酮水平没有影响。这些结果表明，卵巢的交感神经供应在调节卵泡发育和卵巢功能中很重要。这些结果对于进一步了解卵巢发育和功能的神经内分泌调节至关重要，尽管其机制有待未来研究阐明。
