基底样鳞状细胞癌中人乳头瘤病毒的筛查：p16（INK4a）、原位杂交和聚合酶链反应的应用

Screening for human papillomavirus in basaloid squamous carcinoma: utility of p16(INK4a), CISH, and PCR.

作者信息

Winters Ryan, Trotman Winifred, Adamson Christine S C, Rajendran Vanitha, Tang Alice, Elhosseiny Abdelmonem, Evans Mark F

机构信息

Department of Otolaryngology, Tulane University, New Orleans, LA 70115, USA.

出版信息

Int J Surg Pathol. 2011 Jun;19(3):309-14. doi: 10.1177/1066896910380683. Epub 2010 Aug 25.

DOI:10.1177/1066896910380683

PMID:20798066

Abstract

This study compares p16( INK4a) immunohistochemistry (IHC), HPV chromogenic in situ hybridization (ISH), and HPV polymerase chain reaction (PCR) genotyping for detection of HPV infection in basaloid squamous carcinoma (BSCC). A retrospective histopathological analysis of 40 BSCC from a single institution was carried out. p16 IHC, HPV DNA extraction and ISH, and HPV PCR genotyping were performed, and there was excellent agreement between all 3 methods of HPV detection. Analysis of variance yielded no significant differences between the results of the 3 tests ( P = .354) and Pearson product-moment correlation coefficients calculated for each pair of tests demonstrated direct correlation (r = .61 for PCR and IHC, r = .61 for PCR and ISH, and r = 1.00 for ISH and IHC). This supports the use of p16(INK4a) IHC as an initial screening tool for HPV infection in BSCC, while definitive evidence of HPV DNA can be sought subsequently with PCR or CISH.

摘要

本研究比较了p16（INK4a）免疫组织化学（IHC）、人乳头瘤病毒（HPV）显色原位杂交（ISH）和HPV聚合酶链反应（PCR）基因分型在检测基底样鳞状细胞癌（BSCC）中HPV感染的情况。对来自单一机构的40例BSCC进行了回顾性组织病理学分析。进行了p16 IHC、HPV DNA提取与ISH以及HPV PCR基因分型，并且在所有3种HPV检测方法之间存在高度一致性。方差分析显示3项检测结果之间无显著差异（P = 0.354），为每对检测计算的Pearson积矩相关系数显示为直接相关（PCR与IHC的r = 0.61，PCR与ISH的r = 0.61，ISH与IHC的r = 1.00）。这支持将p16（INK4a）IHC用作BSCC中HPV感染的初始筛查工具，而随后可通过PCR或显色原位杂交（CISH）寻求HPV DNA的确切证据。