Mellin Dahlstrand Hanna, Lindquist David, Björnestål Linda, Ohlsson Ann, Dalianis Tina, Munck-Wikland Eva, Elmberger Göran
Department of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, S-171 76 Stockholm, Sweden.
Anticancer Res. 2005 Nov-Dec;25(6C):4375-83.
Human papillomavirus (HPV) in tonsillar carcinoma is correlated with favourable clinical outcome. Here, p16(INK4A), in situ HPV DNA hybridisation (ISH) and HPVL1 capsid detection were evaluated in tonsillar carcinoma to predict the response to radiotherapy (RT) and prognosis.
Fifty-one pre-treatment paraffin-embedded tonsillar cancer biopsies were analysed. Immunohistochemistry (IHC) was used for p16(INK4A) and HPVL1 capsid analysis and PCR and ISH for HPV detection.
High-risk HPV DNA was detected by PCR in 49% of the tumours. P16(INK4a) staining was correlated to HPV In the high-grade p16(INK4a) staining group, 94% had a complete RT response. High p16(INK4a) staining as well as the HPV PCR-positive cases had a favourable prognosis. HPV DNA ISH and L1 IHC could not predict RT response or clinical outcome.
P16(INK4a) overexpression was correlated to HPV in tonsillar carcinoma and is useful for predicting RT response and prognosis in tonsillar carcinoma patients.
扁桃体癌中的人乳头瘤病毒（HPV）与良好的临床结果相关。在此，对扁桃体癌中的p16（INK4A）、原位HPV DNA杂交（ISH）和HPVL1衣壳检测进行评估，以预测放疗（RT）反应和预后。
分析了51例治疗前石蜡包埋的扁桃体癌活检标本。免疫组织化学（IHC）用于p16（INK4A）和HPVL1衣壳分析，PCR和ISH用于HPV检测。
49%的肿瘤通过PCR检测到高危HPV DNA。p16（INK4a）染色与HPV相关。在高等级p16（INK4a）染色组中，94%有完全的放疗反应。高p16（INK4a）染色以及HPV PCR阳性病例有良好的预后。HPV DNA ISH和L1 IHC不能预测放疗反应或临床结果。
p16（INK4a）过表达与扁桃体癌中的HPV相关，可用于预测扁桃体癌患者的放疗反应和预后。
