Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Nanotechnology. 2010 Sep 24;21(38):385101. doi: 10.1088/0957-4484/21/38/385101. Epub 2010 Aug 27.
Development of RNA interference (RNAi) technology utilizing short interfering RNA sequences (siRNA) has focused on creating methods for delivering siRNAs to cells and for enhancing siRNA stability in vitro and in vivo. Here, we describe a novel approach for siRNA cellular delivery using siRNA coiling into carboxyl-functionalized single-wall carbon nanotubes (SWCNTs). The CNT-siRNA delivery system successfully demonstrates nonspecific toxicity and transfection efficiency greater than 95%. This approach offers the potential for siRNA delivery into different types of cells, including hard-to-transfect cells, such as neuronal cells and cardiomyocytes. We also tested the CNT-siRNA system in a non-metastatic human hepatocellular carcinoma cell line (SKHep1). In all types of cells used in this work the CNT-siRNA delivery system showed high efficiency and apparent no side effects for various in vitro applications.
利用短干扰 RNA 序列 (siRNA) 的 RNA 干扰 (RNAi) 技术的发展集中在开发将 siRNA 递送到细胞的方法以及提高 siRNA 在体外和体内的稳定性上。在这里，我们描述了一种利用 siRNA 卷曲成羧基功能化单壁碳纳米管 (SWCNTs) 的新型 siRNA 细胞递送方法。该 CNT-siRNA 递送系统成功地证明了非特异性毒性和转染效率大于 95%。这种方法为将 siRNA 递送到不同类型的细胞提供了可能，包括难以转染的细胞，如神经元细胞和心肌细胞。我们还在非转移性人肝癌细胞系 (SKHep1) 中测试了 CNT-siRNA 系统。在本工作中使用的所有类型的细胞中， CNT-siRNA 递送系统显示了高效率，并且对于各种体外应用没有明显的副作用。
