Universität Hohenheim, Institut für Lebensmittelchemie, Garbenstr. 28, D-70593 Stuttgart, Germany.
Phytochem Anal. 2010 Nov-Dec;21(6):582-9. doi: 10.1002/pca.1238. Epub 2010 Aug 26.
The plant cuticle is a thin, predominantly lipid layer that covers all primary aerial surfaces of vascular plants. The monomeric building blocks of the cutin biopolymer are mainly ω-hydroxy fatty acids.
Analysis of ω-hydroxy fatty acids from cutin isolated from tomato fruits at different stages of decomposition in soil. Different derivatives and mass spectrometric techniques were used for peak identification and evaluation.
Preparation of purified cutin involving dewaxing and HCl treatment. Incubation of purified cutin for 20 months in soil. Pentafluorobenzoyl derivatives were used for GC/MS operated in the electron capture negative ion (ECNI) mode and trimethylsilyl ethers for GC/MS operated in the electron ionisation (EI) mode for analysis of ω-hydroxy fatty acids.
Six ω-hydroxy fatty acids were detected in the purified cutin, three of which were identified as degradation products of 9,16-dihydroxyhexadecanoic acid as a consequence of the HCl treatment involved in the purification step. Incubation of the isolated cutin in soil was accompanied with decrease in concentration of all hydroxyl fatty acids.
We produced evidence that the HCl treatment only affected free hydroxyl groups and thus could be used for proportioning free and bound OH-groups on cutin fatty acids. The method enabled a direct quantification of the ω-hydroxy fatty acids throughout the incubation phase.
植物角质层是一层薄的、主要由脂质组成的层，覆盖维管植物所有的初级气生表面。角质生物聚合物的单体构建块主要是 ω-羟基脂肪酸。
分析在土壤中分解的不同阶段的番茄果实中分离的角质中的 ω-羟基脂肪酸。使用不同的衍生物和质谱技术进行峰识别和评估。
涉及脱蜡和 HCl 处理的纯化角质的制备。将纯化的角质在土壤中孵育 20 个月。使用五氟苯甲酰衍生物进行 GC/MS 在电子俘获负离子 (ECNI) 模式下运行，使用三甲基硅醚进行 GC/MS 在电子电离 (EI) 模式下运行，用于分析 ω-羟基脂肪酸。
在纯化的角质中检测到六种 ω-羟基脂肪酸，其中三种被鉴定为 9,16-二羟基十六烷酸的降解产物，这是由于纯化步骤中涉及的 HCl 处理。在土壤中孵育分离的角质伴随着所有羟基脂肪酸浓度的降低。
我们提供了证据表明 HCl 处理仅影响游离羟基，因此可用于分配角质脂肪酸上的游离和结合 OH 基团。该方法能够在整个孵育阶段直接定量 ω-羟基脂肪酸。
