Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Chemistry. 2010 Oct 11;16(38):11554-7. doi: 10.1002/chem.201001638. Epub 2010 Aug 26.
Quenched: Perylenediimide (PDI) is highly quenched by nucleobases, which greatly restricts its application as a fluorescent probe. Here, we propose "insulator base pairs" tethering cyclohexane ring through D-threoninol. When "insulator base pairs" were inserted between PDI and nucleobases, the quantum yield of PDI drastically increased several thousand-fold. The "insulator base pairs" reported here also have the potential to increase the quantum yields of other fluorophores.
并五苯二酰亚胺（PDI）极易被碱基猝灭，这极大地限制了其作为荧光探针的应用。在这里，我们提出了通过 D-苏糖醇连接环己烷环的“绝缘碱基对”。当“绝缘碱基对”插入 PDI 和碱基之间时，PDI 的量子产率急剧增加了数千倍。这里报道的“绝缘碱基对”也有可能提高其他荧光团的量子产率。
