Matsumoto T, Sakura N, Ueda K
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Pediatr Dermatol. 1990 Dec;7(4):266-9. doi: 10.1111/j.1525-1470.1990.tb01022.x.
X-linked ichthyosis is generally diagnosed by a deficiency of steroid sulfatase activity in fibroblasts or leukocytes. We established a method of assaying nail steroid sulfatase activity for diagnostic use. Nail samples were easy to collect and handle, and satisfied the screening criteria of accuracy, sensitivity, and stability. The detergents Tween 20 and Triton-X 100, which enhance nail STS activity, enabled us to assay the activity with small amounts of nails. The detergent-facilitated assay was also suitable for use with pediatric patients, from whom small amounts of nails were collected.
X连锁鱼鳞病通常通过成纤维细胞或白细胞中类固醇硫酸酯酶活性缺乏来诊断。我们建立了一种用于诊断的测定指甲类固醇硫酸酯酶活性的方法。指甲样本易于采集和处理，并且满足准确性、敏感性和稳定性的筛查标准。可增强指甲类固醇硫酸酯酶（STS）活性的去污剂吐温20和曲拉通X-100，使我们能够用少量指甲测定活性。这种去污剂辅助测定法也适用于采集少量指甲的儿科患者。
