Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education of China, Department of Chemistry, Nanjing University, Hankou Road 22, Nanjing 210093, China.
Talanta. 2010 Sep 15;82(4):1462-7. doi: 10.1016/j.talanta.2010.07.018. Epub 2010 Jul 16.
A novel system of series-wound immunosensing channels (SWIC) was proposed for automated chemiluminescent (CL) dual-analyte immunoassay by immobilizing respectively different capture antibodies on the inner walls of series-wound glass channels. This system could use a single enzyme as label to perform multiplex immunoassay in one fluid way. Using alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as model analytes, the mixture including AFP, horseradish peroxidase (HRP)-labeled anti-AFP antibody, CEA and HRP-labeled anti-CEA antibody was introduced into the SWIC for carrying out the on-line incubation. Upon injection of CL substrate the CL signals from the two immunosensing channels were conveniently resolved and near-simultaneously collected with the aid of optical shutter. AFP and CEA could be rapidly assayed in the ranges of 1.0-100 and 1.0-80 ng/ml with detection limits of 0.41 and 0.39 ng/ml, respectively. The assay results of clinical serum samples were in an acceptable agreement with the reference values. This designed flow-through immunosensing system based on SWIC provided an automated, reusable, simple, sensitive and low-cost approach for multianalyte immunoassay.
提出了一种串联免疫传感通道（SWIC）的新系统，用于通过将不同的捕获抗体分别固定在串联式玻璃通道的内壁上来实现自动化学发光（CL）双分析物免疫分析。该系统可以使用单一酶作为标记物，以单一流体方式进行多重免疫分析。以甲胎蛋白（AFP）和癌胚抗原（CEA）为模型分析物，将包含 AFP、辣根过氧化物酶（HRP）标记的抗 AFP 抗体、CEA 和 HRP 标记的抗 CEA 抗体的混合物引入 SWIC 进行在线孵育。在注入 CL 底物后，借助光学快门方便地解析来自两个免疫传感通道的 CL 信号，并进行近同时采集。AFP 和 CEA 的检测范围分别为 1.0-100 和 1.0-80ng/ml，检测限分别为 0.41 和 0.39ng/ml。临床血清样本的测定结果与参考值具有良好的一致性。基于 SWIC 的这种设计的流通式免疫传感系统为多分析物免疫分析提供了一种自动化、可重复使用、简单、灵敏且低成本的方法。
