Lin Jiehua, Yan Feng, Ju Huangxian
Department of Chemistry, Institute of Analytical Science, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China.
Clin Chim Acta. 2004 Mar;341(1-2):109-15. doi: 10.1016/j.cccn.2003.11.014.
Recently, many automated immunoassay analyzers have been developed for carcinoembryonic antigen (CEA) to overcome the shortcomings in traditional immunoassay methods that are time-consuming and labor-intensive. Flow injection immunoassay (FIIA) has been increasingly applied to laboratory medicine due to its ease in automation, rapid speed and reproducible results. It is important to develop a FIIA method for CEA determination.
Based on a noncompetitive immunoassay format, a CEA-immobilized immunoaffinity column inserted in the flow system was used to trap the unbound horseradish peroxidase (HRP)-labeled antibody after an off-line incubation of CEA and HRP-labeled anti-CEA. The trapped enzyme conjugate was detected by injecting substrates to produce an enhanced chemiluminescence (CL).
The linear range for CEA was 1.0-25 ng/ml with a correlation coefficient of 0.997 and a detection limit of 0.5 ng/ml. The sampling and chemiluminescence detection time for one sample was 5 min after a preincubation procedure of 25 min. Twenty five human serum samples detected by this method were in good agreement with the results obtained by immunoradiometric assay (IRMA).
This method could be used for rapid analysis of CEA and potentially other antigens.
近年来，已开发出多种用于癌胚抗原（CEA）检测的自动化免疫分析仪器，以克服传统免疫分析方法耗时且劳动强度大的缺点。流动注射免疫分析（FIIA）因其易于自动化、速度快且结果可重复，已在检验医学中得到越来越广泛的应用。开发一种用于CEA测定的FIIA方法具有重要意义。
基于非竞争性免疫分析模式，在CEA与辣根过氧化物酶（HRP）标记的抗CEA进行离线孵育后，将插入流动系统中的固定有CEA的免疫亲和柱用于捕获未结合的HRP标记抗体。通过注入底物产生增强化学发光（CL）来检测捕获的酶结合物。
CEA的线性范围为1.0 - 25 ng/ml，相关系数为0.997，检测限为0.5 ng/ml。在25分钟的预孵育程序后，一个样品的采样和化学发光检测时间为5分钟。用该方法检测的25份人血清样品结果与免疫放射分析（IRMA）所得结果高度一致。
该方法可用于CEA以及其他潜在抗原的快速分析。
