Hilakivi-Clarke L A, Durcan M J, Lister R G, Linnoila M
Laboratory of Clinical Studies, DICBR, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Pharmacol Biochem Behav. 1990 Oct;37(2):273-6. doi: 10.1016/0091-3057(90)90333-d.
The effect of tryptophan on immobility in Porsolt's swim test was studied in male NIH Swiss mice. Preexposure to a swim or fight-stressor was included in the design. Doses of tryptophan (0, 12.5, 50, 75, 100, 125 and 200 mg/kg) were administered intraperitoneally 60 min prior to the swim test. In the nonstressed mice tryptophan had an U-shaped dose-response relationship: immobility in the water was dose-dependently shortened after doses from 0 to 100 mg/kg, whereas after 125 and 200 mg/kg tryptophan the immobility times did not differ from the values obtained after a saline injection. Preexposure to a swim- or fight-stressor did not make mice more sensitive to the effects of tryptophan. Tryptophan (0-300 mg/kg) had no effect on exploratory behavior or locomotor activity in the holeboard, suggesting that sedation was not a factor in the swim test results. The findings suggest that tryptophan has antidepressant -like properties in Porsolt's swim test.
在雄性 NIH 瑞士小鼠中研究了色氨酸对波索尔特游泳试验中不动时间的影响。实验设计中纳入了预先暴露于游泳或争斗应激源的环节。在游泳试验前 60 分钟腹腔注射不同剂量的色氨酸（0、12.5、50、75、100、125 和 200 mg/kg）。在未受应激的小鼠中，色氨酸呈现出 U 形剂量反应关系：从 0 至 100 mg/kg 剂量的色氨酸可使水中不动时间剂量依赖性缩短，而 125 和 200 mg/kg 色氨酸处理后的不动时间与注射生理盐水后的数值无差异。预先暴露于游泳或争斗应激源并未使小鼠对色氨酸的作用更敏感。色氨酸（0 - 300 mg/kg）对旷场试验中的探究行为或自发活动无影响，这表明镇静并非游泳试验结果的影响因素。研究结果表明，在波索尔特游泳试验中色氨酸具有类抗抑郁特性。
