阿佐塞米对小鼠离体灌注的髓袢升支粗段的作用。

Effect of azosemide on the in vitro perfused thick ascending limb of Henle's loop from the mouse.

作者信息

Tsuchiya K, Sasaki S, Marumo F

机构信息

Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Pharmacology. 1990;41(4):195-9. doi: 10.1159/000138718.

DOI:10.1159/000138718

PMID:2080229

Abstract

Direct effect of azosemide on the mouse thick ascending limb of Henle's loop was examined by the isolated tubules perfusion technique, and the effect was compared with that of furosemide. Azosemide added to the tubular lumen reversibly suppressed the lumen positive potential (PDt) and decreased the net chloride reabsorption. Dose-response studies of these two drugs suggested that azosemide was about 5 times as potent as furosemide. The more potent effect of azosemide compared with furosemide was confirmed by a paired study in the same tubules. At a concentration of 10(-5) mol/l azosemide suppressed PDt by 91.4%, furosemide by 65.6%.

摘要

采用离体肾小管灌注技术研究了阿佐塞米对小鼠髓袢升支粗段的直接作用，并与呋塞米的作用进行了比较。向肾小管腔中加入阿佐塞米可可逆地抑制管腔正电位（PDt）并减少氯化物的净重吸收。这两种药物的剂量反应研究表明，阿佐塞米的效力约为呋塞米的5倍。在同一肾小管中进行的配对研究证实了阿佐塞米比呋塞米具有更强的作用。在浓度为10^(-5)mol/L时，阿佐塞米使PDt降低了91.4%，呋塞米使PDt降低了65.6%。