Cardoso Nancy, Pandolfi Matías, Ponzo Osvaldo, Carbone Silvia, Szwarcfarb Berta, Scacchi Pablo, Reynoso Roxana
Laboratorio de Endocrinología, Departamento de Fisiología, Facultad de Medicina Universidad de Buenos Aires, Buenos Aires, Argentina.
Neuro Endocrinol Lett. 2010;31(4):512-6.
The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats.
Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA.
Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001).
Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis.
本文旨在研究谷氨酸（GLU）作为双酚A（BPA）在下丘脑水平发挥作用的介质的潜在作用及其对青春期前雄性大鼠生殖轴的影响。
将交配后的Wistar大鼠在饮用水中分别给予0.1%乙醇（对照组，n = 10）或双酚A（双酚A组，n = 10），直至其后代在21日龄断奶。估计母鼠的平均暴露剂量约为2.5毫克/千克体重/天的双酚A。在青春期前阶段（35日龄），处死雄性大鼠，分别通过放射免疫分析（RIA）和高效液相色谱法（HPLC），在含有中基底和视前区前部（MBH - APOA）的下丘脑样本中测量促性腺激素释放激素（Gn - RH）和谷氨酸（GLU）的释放，GLU是一种参与Gn - RH分泌的氨基酸。通过RIA测量血清促黄体生成素（LH）、促卵泡生成素（FSH）水平，通过电化学发光免疫分析（EQLIA）测量睾酮水平。
暴露于双酚A的动物中，Gn - RH和GLU的释放显著降低（p < 0.001，p < 0.01）。治疗后血清LH、FSH和睾酮水平也降低（p < 0.0001）。
目前的结果提供了证据，表明双酚A可能在下丘脑水平起作用，降低GLU的释放，这反过来可能改变Gn - RH的分泌，从而改变轴的正常功能。
